Table 1 Summary table of binding affinities between various CagA constructs and PAR1b.
GST-CagA constructs | Type of CM motif | CM sequence | No. of CM motifs | PAR1b binding | KD ± SE (nM) |
|---|---|---|---|---|---|
∆CM | — | — | 0 | N | ND |
1CMW1 | Western | FPLKRHDKVDDLSKVG | 1 | Y | 90.3 ± 14.1 |
2CMW | Western | FPLKRHDKVDDLSKVG | 2 | Y | 26.6 ± 3.7 |
4CMW | Western | FPLKRHDKVDDLSKVG | 4 | Y | 2.43 ± 0.45 |
1CMW2 | Western | FPLKRHDKVDDLSKVG | 1 | Y | 106 ± 19 |
1CME | East Asian | FPLRRSAAVNDLSKVG | 1 | Y | 46.4 ± 7.4 |
1CMAmI | Amerindian I | SSLKRHAKVDDLSKVG | 1 | N | ND |
1CMAmII | Amerindian II | YTLKMHAGDDNLRSKVG | 1 | N | ND |
