Table 2 The primers used in this study.
Primer | Sequence (5’-3’) | Expected Size | Roles | Source |
|---|---|---|---|---|
Primer used for clone DNA from H. minnesotensis | ||||
 5Sac-FP | CGAGCTCCTGGTAAAATCTGTCCCG | 968 bp | Amplified 5′ UTR and partial sequence of HIM-SKN7 | This study |
 5Xma-RP | CCCCGGGCTGTCGGATAAAACTGGA | This study | ||
 3Asc-FP | GGCGCGCCAATAAGGTGATGAAGCAA | 987 bp | Amplified 3′ UTR and partial sequence of HIM-SKN7 | This study |
 3Pac-RP | CTTAATTAAACCCAATGCGGCACAAGC | This study | ||
 SKN7OX-F | TCTCAAGGTAGCGGCAACAAT | 1.8 kb | Amplified HIM-SKN7 ORF for over-expression and to confirm replacement with hph cassete | This study |
 SKN7OX-R | CCCGCACTGTCTCCATCGTGT | This study | ||
Primer used for identification of null mutants | ||||
 5-HD-FP | GCCGAGGACTTCACCACTA | 741 bp | Amplified HIM-SKN7 gene (PCR 1) | This study |
 3-HD-RP | GCAGCCCATTGACTTGAGA | This study | ||
 HD-F5N | GGCAGTTCGTGGTTTAGTCG | 2.2 kb | Amplified sequence of UTR and partial sequence of hph gene (PCR 2) | This study |
 Hyg-R3 | CTTTGTAGAAACCATCGGCG | This study | ||
 Hyg-NF | AAGTTCGACAGCGTCTCC | 937 bp | Amplified the gene hph (PCR 3) | This study |
 Hyg-NR | TTCCACTATCGGCGAGTA | This study | ||
Primer used for identification of over-expression mutants | ||||
 PromSP3 | AGGTAAGTGAACGACCCGGTC | 1891 bp | Amplifying ORF and partial sequence of PtrpC (PCR4) | This study |
 SKN7OX-R | CCCGCACTGTCTCCATCGTGT | This study | ||
 Hyg-NF | AAGTTCGACAGCGTCTCC | 937 bp | Amplified the gene hph (PCR 5) | This study |
 Hyg-NR | TTCCACTATCGGCGAGTA | This study | ||
Primer used for RT-PCR and RT-qPCT | ||||
 HRT-F | ACCTAAGCCCATACAACG | 254 bp | mRNA transcription of HIM-SKN7 gene | This study |
 HRT-R | TAAATCATTCATCGGGTCAT | |||
 HIM-08F | GGAAACCCAGAAACGACCCT | 149 bp | mRNA transcription of HIM_00008 gene | This study |
 HIM-08R | AGTCGGGTTCGGCTTGTTGT | |||
 HIM-29F | TTCTGTATGGGCTGCGTCTC | 181 bp | mRNA transcription of HIM_00329 gene | This study |
 HIM-29R | TCTGGCTCATCAACAAACCC | |||
 Actin-F | CCCCATCTACGAGGGTTT | 554 bp | Used as internal control | This study |
 Actin-R | GGGAAGCGAGAATGGAAC | |||
