Figure 1
Elevated DNMT1 levels in atherosclerosis-associated macrophages.
(a) mRNA levels of DNMT1, DNMT3a and DNMT3b in adipose tissue-derived macrophages (ATMs) from male C57BL/6 mice fed a normal diet (ND) or a high fat diet (HFD) for 12 weeks (n = 5, *P < 0.05 and ***P < 0.005). (b) mRNA levels of DNMT1, DNMT3a and DNMT3b in the ATMs of male wild-type (WT) or ApoE-knockout (ApoE−/−) mice fed an atherogenic diet for 12 weeks (n = 5, ***P < 0.005). (c) mRNA levels of inflammatory cytokines in the ATMs described in (b) (n = 5, *P < 0.05, **P < 0.01 and ***P < 0.005). (d) Relative mRNA levels of DNMT1 in cultured peritoneal macrophages treated with PBS or LPS (100 ng/ml) for 24 hours (n = 5, **P < 0.01). (e) Immunoblotting assay of DNMT1 in cultured peritoneal macrophages treated with PBS or LPS (100 ng/ml) for 24 hours. (f) Relative mRNA levels of DNMT1 in cultured peritoneal macrophages treated with BSA (5%) or saturated free fatty acid (sFFA, 200 μM) for 24 hours. (g) Immunoblotting assay of DNMT1 in cultured peritoneal macrophages treated with BSA or sFFA (200 μM) for 24 hours.
