Figure 3
Macrophage DNMT1 aggravates proinflammatory cytokine production.
(a) DNMT1 transgene significantly affects the inflammatory pathway and PPAR signaling pathway. Wild-type and DNMT1 transgenic peritoneal macrophages were isolated and treated with LPS (100 ng/ml) for 24 h, followed by a microarray gene assay. Then, the microarray data were subjected to KEGG analysis. The notably altered inflammatory pathways and PPAR signaling pathway, with their corresponding P values, are displayed as indicated. (b) Plasma cytokine levels of the ApoE−/− or macrophage DNMT1 transgenic (TgDNMT1) ApoE−/− mice, which were fed an AD for 12 weeks (n = 6, *P < 0.5, **P < 0.01). (c) mRNA levels of the inflammatory cytokines in the epididymal fat tissues (eFats) isolated from the mice described in (b) (n = 6, **P < 0.01, ***P < 0.005). (d) mRNA levels of the inflammatory cytokines in the livers of the mice described in (b) (n = 6, *P < 0.05 and **P < 0.01). (e) mRNA levels of the inflammatory cytokines in the adipose tissue-derived macrophages (ATMs) from the mice described in (b) (n = 6, *P < 0.05 and **P < 0.01). (f) mRNA levels of the inflammatory cytokines in the arterial plaque-derived macrophages (APMs) from the mice described in (b) (n = 6, **P < 0.01 and ***P < 0.005). (g) mRNA levels of the inflammatory cytokines in the ox-LDL (50 nM)-stimulated peritoneal macrophages (PMs) isolated from the WT or TgDNMT1 mice (n = 6, *P < 0.05 and **P < 0.01). (h) mRNA levels of the inflammatory cytokines in the LPS (100 ng/ml)-stimulated peritoneal macrophages (PMs) isolated from the WT or TgDNMT1 mice (n = 6, *P < 0.05 and **P < 0.01).
