Figure 1 | Scientific Reports

Figure 1

From: A genome-wide imaging-based screening to identify genes involved in synphilin-1 inclusion formation in Saccharomyces cerevisiae

Figure 1

Genome-wide high-content imaging approach to identify components involved in synphilin-1 (SY1) inclusion formation.

(a) Phenotype of WT cells harboring synphilin-1 inclusions. Right panel: dsRed-tagged synphilin-1; Left panel: Bright field. Scale bar, 5 μm. (b) Workflow of the high-content imaging based screening (HCI) to identify mutants altering the synphilin-1 inclusion formation. (c) Software approach for synphilin-1 inclusion identification and quantification. Image pre-processing with shade correction and background subtraction (Top left); Cells (Top right) and inclusion bodies (Bottom left) were isolate from their background based on the signal intensity differences. Then the percentage of cells with inclusions were extracted (Bottom right) by combining the two (cells and inclusion bodies) masks.

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