Figure 2 | Scientific Reports

Figure 2

From: A genome-wide imaging-based screening to identify genes involved in synphilin-1 inclusion formation in Saccharomyces cerevisiae

Figure 2

Top hits showing a decreased percentage of cells with synphilin-1 (SY1) inclusions obtained from the HCI screening.

(a) Images of mutants displaying less synphilin-1 inclusions than WT (his3Δ) cells. Left panel: bright field, right panel: dsRed-synphilin-1. Scale bar, 2 μm. (b) Quantification of the percentage of cells with inclusions in WT and mutant strains. Values are normalized such that the percentage of cells with inclusions in WT is set to 50%. A value of 0% means that there are no cells of that mutant with synphilin-1 inclusions. Statistical analysis was performed using Student’s t-test. Error bars represent standard deviation from triplicates of about 200 cells each. Asterisks denote significant differences between WT and mutants: *P, 0.05; **P, 0.01; ***P, 0.001.

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