Figure 5

Mutants identified from the HCI screening with an increased percentage of cells carrying synphilin-1 (SY1) inclusions.

(a) Representative images of strong hits from the imaging based screening showing cells with expression of dsRed-synphilin-1 or dsRed alone. Left panel, bright field; right panel, dsRed-synphilin-1 or dsRed. Scale bar, 5 μm. (b) Quantification of percentage of cells with inclusions in WT and mutants. Values are normalized such that the percentage of cells with inclusions in WT cells is set to 50%. Error bars represent standard deviation from triplicates of about 200 cells each. Asterisks denote significant differences between WT and mutants (Student’s t-test): *P, 0.05; **P, 0.01; ***P, 0.001. (c) Functional enrichment analysis of mutants displaying increased synphilin-1 inclusion formation. Confirmed mutants were analyzed for enrichment of GO functional categories. Enriched groups were scored by comparing to a background list of SGA-V2+ ts-V5 array. Functional groups are marked with different colors. (d) Network analysis of mutants with an increased percentage of cells with synphilin-1 inclusions. Mutants showing more cells with synphilin-1 inclusions were grouped into modules based on their known physical interactions and published information of the cellular components. The cellular components are shown in different colored nodes. The circles indicate subunits or protein complexes.