Figure 3
From: Deep sequencing reveals global patterns of mRNA recruitment during translation initiation
The regional characteristics of the sequence patterns in the TIR upstream of AUG.
The k-mer analysis was performed to describe the sequence pattern of each mRNA library, (a) N8U, (b) N8M and (c) N8D, selected by different initiation complexes: 30S, 30SIC and 70SIC. As described in the Methods, each 6-mer sequence has a unique coordinate in the 26 × 26 matrix. At each unique coordinate, the red color means that the 6-mer sequence occurred more frequently than in the background (the sequence occurrence of the same 6-mer in the mRNA library before any selection), whereas the blue color means the 6-mer occurred less frequently than in the background. The value in the color bar describes the extent of the difference in frequency.
