Figure 4: Schematic presentation of primer design and detection of IHH deletion by a diagnostic PCR test. | Scientific Reports

Figure 4: Schematic presentation of primer design and detection of IHH deletion by a diagnostic PCR test.

From: Deletion of Indian hedgehog gene causes dominant semi-lethal Creeper trait in chicken

Figure 4

(a) Diagnostic primers for wild-type and Creeper chickens. Expected size of PCR product of the wild-type chicken is 438 bp while for the Creeper chicken is 224 bp. (b) Diagnostic genotyping of three phenotypes. Lethal embryos has a single PCR product of 244 bp, Creeper chicken has two bands at 244 bp and 438 bp, and the wild-type chicken has a single band at 438 bp.

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