Figure 6: Expression analysis of IHH in tibial cartilages in three genotypic individuals.
From: Deletion of Indian hedgehog gene causes dominant semi-lethal Creeper trait in chicken
(a) qPCR analysis of IHH at E4 (embryonic day 4), D1 (day of hatch), and D84 (day 84 post-hatch) (N = 8 for each group). (b,d) Western blot analysis of IHH from Creeper and wild-type male chicks at D1 and D84. (c,e) The quantitative analysis of western blot results at D1 and D84 using Image J software. Western blot analysis showed the expression of IHH protein in chicken tibial cartilages. β-actin was used as a loading control. Three biological replicates were conducted in this experiment. Data are expressed as mean ± SD (N = 3). *p < 0.05.
