Figure 4

Ectopic expression of TaGLDH-A1, -B1 and -D1 homoeologs in N. benthamiana using PEBV viral vector.
PEBV:A1, PEBV:B1 and PEBV:D1 are three recombinant viruses expressing TaGLDH-A1, -B1 and -D1, respectively. PEBV:GUS is another recombinant virus expressing a bacterial GUS gene (encoding β-glucuronidase). (A) Total GLDH activity levels in mock controls and the plants infected by different recombinant viruses. The values displayed are means ± SD of three measurements for three different plants. Different letters above the histograms indicate statistical difference (P < 0.05). (B) Total and reduced AsA contents in mock controls and the plants infected by different recombinant viruses. The contents shown are means ± SD of three measurements of three different plants. Two independent experiments were performed with similar results obtained.