Figure 5 | Scientific Reports

Figure 5

From: A novel allele of L-galactono-1,4-lactone dehydrogenase is associated with enhanced drought tolerance through affecting stomatal aperture in common wheat

Figure 5

Function of TaGLDH in AsA biosynthesis as investigated using BSMV mediated VIGS.

BSMV:GFP, expressing a green fluorescence protein, was used for monitoring viral spread in infected wheat plants, whereas BSMV:GLDHas was for silencing TaGLDH transcripts. (A) Comparison of relative TaGLDH expression levels among different plant samples, with TaGLDH expression in the uninoculated plants set as 1. TaGLDH expression level was assayed by qRT-PCR. Significant decrease of TaGLDH expression was detected in the plants infected by BSMV:GLDHas but not in the mock controls or those infected by BSMV:GFP. (B–D) Significant reductions of TaGLDH activity and the contents of total and reduced AsA in the plants infected by BSMV:GLDHas but not in those infected by BSMV:GFP. The values depicted are all means ± SD of three measurements for three different plants. Different letters above the histograms mark statistical difference (P < 0.05). The assays were repeated at least twice with similar results found.

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