Figure 6 | Scientific Reports

Figure 6

From: A novel allele of L-galactono-1,4-lactone dehydrogenase is associated with enhanced drought tolerance through affecting stomatal aperture in common wheat

Figure 6

Analysis of TaGLDH-A1a and TaGLDH-A1b alleles.

(A) SDS-PAGE and protein blot assays of TaGLDH-A1a and TaGLDH-A1b recombinant proteins. The two alleles were expressed in E. coli cells without the putative mitochondrial targeting peptide but with a histidine tag at the N-terminal end, followed by purification with nickel affinity chromatography and the protein blot assay using an antibody specific for histidine tag. (B,C) Comparison of total TaGLDH activity levels and the contents of total and reduced AsA in Xiaoyan 54 (carrying TaGLDH-A1a) and the three Xinjiang wheat cultivars (Kashi 1, Kashibaipi and Tutoumai, with TaGLDH-A1b). The values are means ± SD of three measurements for three different plants. Different letters above the histograms indicate statistical difference (P < 0.05). For both (B,C), the data displayed are typical of three independent assays.

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