Figure 1

Trabecular bone mass and marrow adiposity.

(a–g) Representative H&E images demonstrating trabecular bone and marrow adipose tissue in the distal femora of mice. Mice were modeled 4 weeks for the control groups (a) and osteoporoses induced by natural aging (b), accelerated senescence (SAMP6) (c), ovariectomy (OVX) (d), type 1 diabetes (T1D) (e), excessive glucocorticoids (GIOP) (f) and orchidectomy (ORX) (g). The unstained area in the bone marrow represented empty spaces occupied by adipocytes with yellow allows indicating. Bars: 100 μm. (h) Analyzed by micro-CT, the parameter of bone mineral density (BMD) in the distal metaphysis of femora demonstrating bone loss in all of the 6 types of osteoporoses, among which natural aging mice exhibited the most severe bone loss. (i–k) In bone histomorphometric analysis by H&E staining, the corresponding parameters of trabecular bone area over total area (i), number of adipocytes over total area (j) and adipocyte area over total area (k) showing osteoporotic bone loss with increased marrow adiposity in 6 different types of osteoporoses, among which natural aging mice developed the most adipocytes. Data represents mean ± standard deviation (SD). n = 6 per group. ^aP < 0.05 with the control groups; ^bP < 0.05 with the natural aging group; ^cP < 0.05 with the SAMP6 group; ^dP < 0.05 with the OVX group; ^eP < 0.05 with the T1D group; ^fP < 0.05 with the GIOP group; ^gP < 0.05 with the ORX group.