Figure 2
Cortical bone formation parameters.
(a–g) Representative calcein labeling images demonstrating cortical bone formation in the cortical endosteum of the distal femora of mice. Mice were modeled 4 weeks for the control groups (a) and osteoporoses induced by natural aging (b), accelerated senescence (SAMP6) (c), ovariectomy (OVX) (d), type 1 diabetes (T1D) (e), excessive glucocorticoids (GIOP) (f) and orchidectomy (ORX). (g) Mice accepted double intraperitoneal injection of 20 mg/kg calcein at 16 days and 2 days prior to sacrifice. Calcein was dissolved in PBS at 2 mg/ml supplemented with 1 mg/ml NaHCO3. Bars: 100 μm. (h–j) The corresponding parameters of mineral apposition rate (h), mineralized surface over bone surface (i) and bone formation rate (j) showing impaired cortical bone formation in 6 different types of osteoporoses. The cortical bone formation of natural aging mice declined the most. Data represents mean ± standard deviation (SD). n = 6 per group. aP < 0.05 with the control groups; bP < 0.05 with the natural aging group; cP < 0.05 with the SAMP6 group; dP < 0.05 with the OVX group; eP < 0.05 with the T1D group; fP < 0.05 with the GIOP group; gP < 0.05 with the ORX group.
