Figure 7
Adipogenic differentiation of bone marrow stromal cells (BMSCs).
(a–g) Representative oil red O staining images demonstrating formation of lipid droplets by murine BMSCs derived from the control groups (a) and osteoporoses induced by natural aging (b), accelerated senescence (SAMP6) (c), ovariectomy (OVX) (d), type 1 diabetes (T1D) (e), excessive glucocorticoids (GIOP) (f) and orchidectomy (ORX) (g). Mice were sacrificed after 4 weeks of modeling. 1st-passaged BMSCs were seeded at 2 × 105 cells/well in 12-well plates and underwent adipogenic induction for 14 days. Bars: 100 μm. (h–j) The corresponding parameters of lipid droplets formation over total area (h), number of lipid droplets per square millimeter (i) and average size of lipid droplets (j) showing stimulated adipogenic differentiation of BMSCs in all of the 6 types of osteoporoses. BMSCs from natural aging mice and the SAMP6 mice developed the largest area of lipid droplets. (k) After 14-day adipogenic induction, quantitative real-time polymerase chain reaction (qRT-PCR) analysis of the mRNA expression level of adipogenic marker gene Peroxisome proliferator activated receptor gamma (PPARγ). The corresponding values showed increased adipogenic differentiation of BMSCs in all of the 6 types of osteoporoses. BMSCs from natural aging mice and the SAMP6 mice expressed the highest level of PPARγ. The expression of PPARγ was normalized to that of ACTIN. Data represents mean ± standard deviation (SD). n = 6 per group. aP < 0.05 with the control groups; bP < 0.05 with the natural aging group; cP < 0.05 with the SAMP6 group; dP < 0.05 with the OVX group; eP < 0.05 with the T1D group; fP < 0.05 with the GIOP group; gP < 0.05 with the ORX group.
