Figure 1: The effects of CA-4 treatment on zebrafish embryos.
From: Combretastatin A-4 efficiently inhibits angiogenesis and induces neuronal apoptosis in zebrafish
(A) Investigation of the suitable time window of 10 ng/ml CA-4 treatment. The phenotypes of embryo dosed at gastrula, segmentation, pharyngula periods were analyzed. The experiments of CA-4 treatment during each time window were repeated in triplicate at least. (B,C) Percentage of phenotype induced by 1–100 ng/ml CA-4 treatment at 8 hpf, analyzed at 36 hpf (B) and 60 hpf (C), respectively. The experiments of CA-4 treatment at each concentration were repeated in triplicate. The percentage of Dead, Malformed, Tardive and Normal was displayed in Grey, Pink, Orange and Green columns, respectively.
