Figure 5: Western blot analysis of expression of SR-B1 and CYP7A1 in mice.
From: Osteopontin Deficiency Alters Biliary Homeostasis and Protects against Gallstone Formation
(a,c) Hepatic proteins were isolated from WT and OPN−/− mice and analysed by Western blotting for SR-B1 and CYP7A1 protein expression. The gender of mice was labelled above the bands. β-Actin was selected as a control for gel loading. (b,d) Quantification of Western blots. The relative average protein level was determined by densitometry. The data are expressed as the mean ± SD (n = 6 per group, 3 female and 3 male mice). Statistical analysis was performed using unpaired Student’s t test, *P < 0.05, **P < 0.01. WT, wild type; OPN−/−, OPN deficient; M, male; F, female.
