Figure 9 | Scientific Reports

Figure 9

From: Protective effect of epigallocatechin-3-gallate (EGCG) via Nrf2 pathway against oxalate-induced epithelial mesenchymal transition (EMT) of renal tubular cells

Figure 9

Si-Nrf2 abrogated the protective effect of EGCG against oxalate-induced EMT.

(A,B) Efficacy of Nrf2 knockdown by si-Nrf2 was confirmed by immunofluorescence staining in the si-Nrf2-transfected cells, whereas the si-Control-transfected cells served as the control. (C,D) ZO-1 expression was evaluated as a representative epithelial marker. (E,F) Vimentin expression was evaluated as a representative mesenchymal marker. (G,H) Catalase, a product of Nrf2 activation, was evaluated by Western blotting (GAPDH served as the loading control). Original magnification power = 1,000X in all panels of (A,C,E). Fluorescence intensities representing Nrf2 (B), ZO-1 (D) and vimentin (F) levels were measured and analyzed from 10 random high-power fields (HPF) and at least 100 cells in each sample. N = 3 independent experiments. *p < 0.05 vs. control; #p < 0.05 vs. oxalate; †p < 0.05 vs. si-Control group.

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