Table 2 Peptide profiling of 120 min duodenal digests of wheat LTP alone and in the presence of 0.26 mM linoleic acid determined by MALDI-ToF mass spectrometry.

LTP fragments	m/z		Normalised relative intensity
LTP fragments	Observed	Calculated	Wheat LTP alone	Wheat LTP + linoleic acid
Intact protein (residues 1–90)	10063.22	10063.18	518	705
1–34	3474.74	3474.93	160	292
1–39	4310.6	4309.77	2546	3290
1–56	6243.58	6242.91	537	1047
1–67	7477.3	7477.18	214	290
1–79	8803.44	8803.75	270	2300
17–39*	2436.75	2436.65	805	1290
17–56	4370.01	4369.78	533	ND
17–61	4904.39	4904.39	90	90
40–56	1951.23	1951.15	292	ND
40–67	3185.43	3185.43	248	340
40–79	4512.11	4512.0	128	656
57–67*	1252.34	1252.29	450	ND
57–89	3739.16	3739.16	110	130
68–79*	1344.71	1344.58	334	ND
80–90*	1277.74	1277.45	1550	1200
Unassigned	3514.0	—	2833	1668
Unassigned	1098.54	—	1320	ND
Unassigned	2679.24	—	1373	1927
Unassigned	4334.4	—	1027	1070
Unassigned	5032.13	—	435	ND
Unassigned	5379.31	—	479	ND
Unassigned	5794.72	—	1004	ND
Unassigned	8673.0	—	312	400

Peptide assignments were derived from comparison of experimentally derived masses with those obtained through in silico digestion of wheat LTP (Uniprot ID P24296, Figure S2) with trypsin and chymotrypsin. Peptide assignments corresponding to the most intense mass events are in bold. Asterisks denote peptides that were confirmed by LC-MS/MS. ND- not detected.

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