Table 2 Global fitting parameters of FCCS data at different Ykt6:DPC ratios.

From: Lipid Regulated Intramolecular Conformational Dynamics of SNARE-Protein Ykt6

Fitting

parameter

Ykt6:DPC = 1:0

Ykt6:DPC = 1:2

N

9.11 ± 0.53

8.87 ± 0.67

τD (μs)

220 ± 37

224 ± 7

τi (μs)

190 ± 55

15 ± 32

α

0.13 ± 0.04

0.04 ± 0.05

β

0.12 ± 0.05

0.07 ± 0.11

γ

1.01 × 10−4 ± 0.91 × 10−4

0.10 × 10−2 ± 0.88 × 10−2

δ

0.25 ± 0.06

0.15 ± 0.06

  1. N is the average number of molecules in the focal region; τD is the average transit time through the focal area; τi represents the time constant for the dynamic among the correlations which is the intramolecular dynamic; parameters α and γ are scaling factors due to the correlation amplitudes; β and δ represent static contributions from FRET states. Compare the τi value between different ration of DPC, it shows that when the ratio increases to 1:2, the value of τi is in the range of the error bound of apo system.
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