Figure 2
(Top) Free Energy profile of the WW domain WT and of the Y65C mutant as a function of the native contact map (CMAP); the shaded region represents the error. (Bottom) Projection of the WT and Y65C folding free energy profile along the contact map and the fraction of β structure of the first (β1β2) and second (β2β3) hairpins. The β1β2 element appears to be the most stable and is well structured also in partially folded conformations (black dotted boxes, CMAP between 10 and 15). The mutant partially folded conformations however, appear less structured, and misfolded conformations (with low CMAP and some residual β structure) appear (red boixes).
