Table 3 Difference in cell composition (median [95% confidence limit]) within pairs of technical replicates and study duplicates (estimated with the Houseman algorithm).

From: Reliability of DNA methylation measures from dried blood spots and mononuclear cells using the HumanMethylation450k BeadArray

Type of duplicates

Type of sample

CD8T difference

CD4T difference

NK difference

Bcell differencea

Monocyte difference

Granulocyte differencea

Technical replicates

PBMC

1.1% [0.0–2.4%]

0.6% [0.1–1.5%]

0.6% [0.1–4.8%]

0.4% [0.1–2.0%]

0.5% [0.1–2.1%]

0.3% [0.1–9.6%]

Technical replicates

DBS

0.9% [0.1–8.3%]

0.5% [0.1–4.9%]

1.0% [0.1–5.0%]

0.8% [0.1–3.3%]

0.9% [0.1–2.6%]

1.0% [0.1–9.8%]

Study duplicates

PBMC

1.9% [0.4–13.1%]

1.4% [0.2–11.5%]

1.4% [0.1–8.5%]

1.2% [0.2–6.7%]

0.9% [0.0–3.3%]

1.5% [0.1–9.1%]

Study duplicates

DBS

1.9% [0.1–7.9%]

1.4% [0.1–6.0%]

1.0% [0.1–3.5%]

0.9% [0.1–3.9%]

0.8% [0.2–3.1%]

2.2% [1.1–7.9%]

  1. aAlthough PBMC samples are mostly made up of lymphocytes and monocytes, the estimates for B-cell and granulocyte counts are not null but lower.
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