Figure 3
From: Addition of lysophospholipids with large head groups to cells inhibits Shiga toxin binding
LPL treatment effects on Stx cell association.
HEp-2 cells were pretreated with LPLs or other substances for 30 min in HEPES-buffered medium without FBS for 30 min at 37 °C, prior to incubation with 125I-Stx1m for 20 min at 37 °C. Cells were washed, lysed and the radioactivity was measured. All panels show quantification data of cell-associated 125I-Stx1m, expressed as % of control. (a) Cells were pretreated with 10 or 20 μM LPLs before incubation with 125I-Stx1m (mean ± SEM; n ≥ 3, except for 20 μM LPS brain and 20 μM LPE brain plasmalogen showing mean ± STD; n = 2). (b) A representative figure for one out of five independent experiments, showing amounts of cell-associated 125I-Stx1m when treated with LPI (bovine liver LPI, mostly 18:0), pure LPI 18:0 and pure LPI 16:0 at increasing concentrations (mean ± STD, duplicates). (c) Quantification of cell-associated 125I-Stx1m after treatment with LPI (bovine liver, mostly 18:0), LPI 18:0 and LPI 16:0 at increasing concentrations (mean ± SEM; n ≥ 5). (d) Quantification of cell-associated 125I-Stx1m after treatment with LPC 18:0 and LPC 18:1 at 10 and 20 μM (mean ± SEM; n = 3). (e) Quantification of cell-associated 125I-Stx1m upon treatment with various LPLs with acyl chains containing mostly 18:0 or pure 18:0 (mean ± SEM; n ≥ 3).
