Figure 2
Legumain overexpression in M2 macrophages is accompanied by angiogenesis and less accumulation of ECM components in the OCI-Ly3 xenograft mouse model of DLBCL.
The OCI-Ly3 xenograft mouse model of DLBCL was established and tumours were collected when the tumour size had reached 0.8 or 1.5 mm3 (n = 5). Changes in the tumour size, ECM deposition, angiogenesis and legumain expression were measured. (A) Tumour growth curve of the OCI-Ly3 xenograft mouse model of DLBCL. (B) Flow cytometry analysis of CD206 vs F4/80 expression in the tumour tissues collected at different stages in the DLBCL animal model. (C) Representative images and statistical results of RT-PCR analyses of legumain mRNA expression at different stages. (D) Representative images of immunofluorescence staining for legumain (green) and CD206 (red). The arrows indicate co-localization of CD206 and legumain. Scale bar = 50 μm. (E,F) Statistical analysis and representative images of immunofluorescence staining for CD31 and Sirius red staining in tumour tissues samples at different stages. Scale bar = 100 μm. Scale bar = 50 μm. Data are shown as the mean ± s.e.m, *P < 0.05 and ***P < 0.001.
