Figure 4 | Scientific Reports

Figure 4

From: Stronger enhancer II/core promoter activities of hepatitis B virus isolates of B2 subgenotype than those of C2 subgenotype

Figure 4

Impact of exchanging core promoter or just nucleotide positions 1633/1635/1636/1652 between B2 and C2 clones on HBV RNA transcription, DNA replication and core protein expression.

The name above the line indicates the original clone and their genotype. The name below indicates the source of core promoter. P1: G1633A/A1635G/A1636T/G1652A. P1′: A1633G/G1635A/T1636A/A1652G. CP: core promoter. Huh7 cells transiently transfected with dimeric HBV DNA constructs were harvested 3 days later. (A) Northern blot of HBV RNAs using GAPDH as a loading control. (B) Southern blot of replicative DNA; (C) Western blot of core protein with GAPDH serving as a loading control.

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