Table 2 Replication and transmission of cold-adapted H9N2 virus in chickens.

Virus	Group	Virus shedding in oropharynx on day ^a		Virus shedding in cloaca on day ^a		Virus replication in tracheas on day ^a				Virus replication in lungs on day ^a
Virus	Group	2	4	2	4	2	3	5	7	2	3	5	7
SD/01/10-wt	Inoculation	6/6 (5.3 ± 0.6)	6/6 (4.9 ± 0.5)	6/6 (3.3 ± 0.7)	4/6 (2.2 ± 0.3)	6/6 (3.4 ± 0.4) ^b	6/6 (4.4 ± 0.4)	6/6 (3.6 ± 0.2)	1/6 (1.5)	6/6 (4.2 ± 0.4)	6/6 (5.3 ± 0.9)	6/6 (3.5 ± 0.3)	1/6 (1.8)
SD/01/10-wt	Contact	6/6 (4.8 ± 0.3)	6/6 (5.2 ± 0.4)	6/6 (3.7 ± 0.2)	2/6 (2.0 ± 0.4)	/	/	/	/	/	/	/	/
SD/01/10-ca	Inoculation	5/6 (1.3 ± 0.5)	0/6	0/6	0/6	6/6 (1.8 ± 0.4)	0/6	0/6	0/6	0/6	0/6	0/6	0/6
SD/01/10-ca	Contact	0/6	0/6	0/6	0/6	/	/	/	/	/	/	/	/

^a3-week-old SPF chickens were inoculated intranasally with 10⁶ EID₅₀ in 0.2 mL of SD/01/10-ca or SD/01/10-wt. At 24 h post inoculation, six naive in-contact chickens were added to each infected group. Six inoculated chickens in each group were killed on day 2, 3, 5, 7 post inoculation and their organs were collected. Oropharyngeal and cloacal swabs of directly infected chickens and in-contact chickens were collected on 2 and 4 days post inoculation. Collected organs and swabs were titrated in eggs.
^bNo. of chickens positive for virus/total no. of chickens tested (mean titers [log₁₀ EID₅₀/mL] ± standard errors).

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