Figure 4: Down-regulation of GILZ expression induced quiescence of dormant DMC-derived B16F1-GFP-D cells in the G0 phase. | Scientific Reports

Figure 4: Down-regulation of GILZ expression induced quiescence of dormant DMC-derived B16F1-GFP-D cells in the G0 phase.

From: Melanoma dormancy in a mouse model is linked to GILZ/FOXO3A-dependent quiescence of disseminated stem-like cells

Figure 4

(a) Cell cycle analysis of dormant DMC-derived B16F1-GFP-D (lower panel) and maternal B16F1-GFP-M (upper panel) cells transfected with control (left) or GILZ-specific (right) siRNA revealed that the dormant DMC-derived cells possessed the novel, GILZ-dependent ability to control the G0-to-G1 transition in addition to the known GILZ-dependent ability to control the G1-to-S transition of the cell cycle. A fraction of cells in the G0 phase of the cell cycle are shown in red. (b) Sphere-forming units (SFUs) formed by B16F1-GFP-D (grey) or B16F1-GFP-M (white) cells transfected with control and GILZ-specific siRNA. (c) qRT-PCR assay of Tsc22d3 expression in spheres obtained from B16F1-GFP-M and B16F1-GFP-D cells transfected with either control or GILZ-specific siRNA and cultured for 7 days. The values indicate the level of GILZ-encoding mRNA relative to the control (B16-F1GFP-M adherent cells) (ΔΔCt); siCTR (control siRNA), siGILZ (GILZ-specific siRNA). The results represent 3 independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001.

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