Figure 6: Down-regulation of GILZ induced regulators of quiescence. | Scientific Reports

Figure 6: Down-regulation of GILZ induced regulators of quiescence.

From: Melanoma dormancy in a mouse model is linked to GILZ/FOXO3A-dependent quiescence of disseminated stem-like cells

Figure 6

(a) Inactivation of the PI3K/AKT pathway via incubation in10 μM LY294002 (LY) for 24 h inhibited Tsc22d3 expression in dormant DMC-derived B16-F1-GFP-D (right) and B16-F1-GFP-M cells (left) **p < 0.01 (n = 3). (b) Western blot analysis of relevant proteins affected by GILZ down-regulation in the maternal and dormant cell-derived cell lines; pFOXO3A denotes phosphorylated FOXO3A. (c) Down-regulation of GILZ affects the subcellular localisation of quiescence-related factors. Representative immunofluorescence images generated using anti-p21CIP1 and anti-FOXO3A (red) antibodies are shown. Hoechst 33342 (blue) was used for nuclear counterstaining. (d) Quantification by counting of the immunostained nuclei shown in (c); maternal cells (white), dormant cells (grey), GILZ KD (stripes); 4–15 fields were counted by 3 independent investigators. *p < 0.05; ***p < 0.001.

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