Figure 7: Down-regulation of GILZ induced cellular quiescence of human melanoma in vitro and in vivo. | Scientific Reports

Figure 7: Down-regulation of GILZ induced cellular quiescence of human melanoma in vitro and in vivo.

From: Melanoma dormancy in a mouse model is linked to GILZ/FOXO3A-dependent quiescence of disseminated stem-like cells

Figure 7

Human melanoma HBL-H2B-GFP cells were treated with tetracycline to induce the cell division-tracking H2B-GFP protein, a marker of quiescence, and were subsequently transfected transiently with control (siCTR) or GILZ-specific (siGILZ) siRNA. (a) qRT-PCR analysis of GILZ expression in FACS-sorted melanoma GFPlow fast-cycling cells and GFPhigh quiescent cells. *p < 0.05 (n = 2). (b) Flow cytometry analysis of the cell cycle in control (siCTR) and GILZ down-regulated (siGILZ) human melanoma cells. (c) Immunoblots with an anti-phosphorylated (S253) FOXO3A (pFOXO3A) antibody after transfection of human melanoma HBL-H2B-GFP cells with control or human GILZ-specific siRNA. (d) Graphs showing the changes in the sphere-forming units (SFUs) and colony-forming units (CFUs) of human melanoma HBL cells after the down-regulation of GILZ expression. ***p < 0.001, **p < 0.01 (n = 3). (e) qRT-PCR analysis of relative TYROSINASE and TSC22D3 expression in 6 human melanoma samples obtained from patients and in human melanoma HBL H2B-GFP cells treated with siCTR and siGILZ.

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