Figure 1 | Scientific Reports

Figure 1

From: Human DNA-Damage-Inducible 2 Protein Is Structurally and Functionally Distinct from Its Yeast Ortholog

Figure 1

Sequence analysis of Ddi1 orthologs.

(A) Sequence alignment of Ddi1-like proteins from various eukaryotic organisms. Domains are indicated with double-headed arrows. The highly conserved catalytic site of RVP is highlighted. The putative UIM motif is highlighted in bold, with residues important for ubiquitin binding in red. (B) Schematic diagram of full-length hDdi2 and the truncated constructs used in this study. Positions of the histidine tag including the factor Xa cleavage site (green), UBL domain (yellow), HDD (gray), RVP domain (orange) and C-terminal UIM (black helix) are indicated. Flexible regions are indicated with blue boxes. Mutation of the putative catalytic aspartate (D252A) is indicated with a red arrow.

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