Figure 2
Mapping of the UBQ-hDdi2 interaction site.
(A) 15N/1H-HSQC titration spectra of UBQ with hDdi2-UIM peptide. (B) Identification of mapped residues shown on the UBQ structure (PDB entry 1D3Z)32. (C) Titration curves of selected amino acids on UBQ. (D) Plot of chemical shift perturbations of individual amino acids upon interaction at the end point of the titration (35-fold molar excess). Red crosses mark amino acids that were not reliably observed in the titration spectra. (E) Plots of chemical shift perturbations of UBQ residues upon interaction with 2.2 mM hDdi2-UIM peptide (blue) and upon addition of hDdi2-scrambled UIM peptide (red) to a final concentration of 1.9 mM.
