Figure 1
From: Thymic DCs derived IL-27 regulates the final maturation of CD4+ SP thymocytes
IL-27 and its receptor expression in the thymus.
(A) Total thymocyte, CD11cintCD45RA+pDC, CD11chiCD8α+CD172α− and CD11chiCD8α−CD172α+ cDC, CD45−Epcamhi Ly51+ cTEC, CD45-Epcamhi Ly51- mTEC and CD11b+F4/80+ macrophage were isolated from C57BL/6J mice. The expression of p28 and EBI3 mRNA was evaluated by quantitative PCR. nd, no detectable. (B) IL-27 protein expression in pDC, CD8α+ and CD172α+ cDC was analyzed by flow cytometry following intracellular staining with anti-IL-27. Representative histograms are shown on the left and the mean fluorescence intensity (MFI) on the right. (C) Purified DN, DP, CD8 SP and the four subsets of CD4 SP (SP1-4) thymocytes were analyzed for IL-27RA and gp130 mRNA expression using quantitative PCR. The lymph node (LN) naïve T cell was used as a positive control. All the experiments were repeated for more than three times and the data are shown as mean ± s.d. *p < 0.05; **p < 0.01.
