Figure 3
From: Thymic DCs derived IL-27 regulates the final maturation of CD4+ SP thymocytes
Defect in thymocyte development in CD11c-cre p28flox/flox mice.
Single cell suspension was prepared from the thymus of CD11c-cre p28flox/flox mice and littermate controls. T cell development was analyzed by flow cytometry. (A) Representative dot plots showing CD4/CD8 staining of total thymocytes (upper) and CD44/CD25 staining of cells in the CD45+CD4−CD8− gate (lower). (B) Representative dot plots showing 6C10/CD69 (upper) and CD69/Qa-2 (lower) staining of CD4+CD8−CD25−NK1.1− thymocytes. (C,D) The percentage (C) and absolute cell number (D) of the four subsets of CD4 SP thymocytes subsets in CD11c-cre p28flox/flox mice and littermate. Data were collected from 12 pairs of mice. Each dot or square represents an individual mouse and the horizontal bar denotes the mean. *p < 0.05; **p < 0.01.
