Figure 4
OGD-induced apoptosis attenuated by IL-10 via JAK1/STAT3 pathway.
(A) Twenty-four hours after OGD, apoptosis of neurons were detected by flow cytometry. The signals from apoptotic neurons were localized in the Q2 and Q4 quadrants of the resulting dot-plot graph. (a) Control group; (b) OGD group; (c) OGD + IL-10NA group; (d) OGD + IL-10 + GLPG0634 group; (e) OGD + IL-10 group (f) Statistical graph of apoptosis in different groups (n = 3). *p < 0.001, as compared with control group; #p < 0.01, as compared with OGD group; ##p < 0.01, as compared with OGD group; **p < 0.05, as compared with OGD + IL-10 group; by one way analysis of variance (ANOVA) followed by Student-Newman-Keuls multiple comparisons test, F = 28.579, p < 0.0001. (B) qPCR analysis of Bax (n = 4). Control group was set as calibrator sample representing the 1 × expression. *p < 0.01, as compared with control group; #p < 0.001, as compared with OGD group; ##p < 0.05, as compared with OGD group; **p < 0.05, as compared with OGD + IL-10 group; by one way analysis of variance (ANOVA) followed by Student-Newman-Keuls multiple comparisons test, F = 42.490, p < 0.0001. (C) qPCR analysis of Bcl-2 (n = 3). Control group was set as calibrator sample representing the 1 × expression. *p < 0.001, as compared with control group; #p < 0.01, as compared with OGD group; ##p < 0.001, as compared with OGD group; **p < 0.001, as compared with OGD + IL-10 group; by one way analysis of variance (ANOVA) followed by Student-Newman-Keuls multiple comparisons test, F = 86.127, p < 0.0001. (D) The representative image of western blot analysis for Bax and Bcl-2 expression. (E) Western blot analysis of Bax (n = 4). *p < 0.001, as compared with control group; #p < 0.01, as compared with OGD group; ##p < 0.01, as compared with OGD group; **p < 0.01, as compared with OGD + IL-10 group; by one way analysis of variance (ANOVA) followed by Student-Newman-Keuls multiple comparisons test, F = 36.711, p < 0.0001. (F) Western blot analysis of Bcl-2 (n = 4). *p < 0.01, as compared with control group; #p < 0.001, as compared with OGD group; ##p < 0.05, as compared with OGD group; **p < 0.01, as compared with OGD + IL-10 group; by one way analysis of variance (ANOVA) followed by Student-Newman-Keuls multiple comparisons test, F = 33.323, p < 0.0001. All data are presented as mean ± SEM.
