Figure 5
Detection of neurites by immunofluorescence.
(A) Cyton, neurites (red) and nucleus (blue). (B) Manually tracing the length of the longest neurite (white) with the LSM (4.2.0.121) software. (C) Number of primary neurites (arrows) was calculated. (D) Statistical analysis of the longest neurite length (n = 3). *p < 0.001, as compared with control group; #p < 0.001, as compared with OGD group; ##p < 0.001, as compared with OGD group; **p < 0.001, as compared with OGD + IL-10 group; by one way analysis of variance (ANOVA) followed by Student-Newman-Keuls multiple comparisons test, F = 128.113, p < 0.0001. (E) Statistical analysis of the number of primary neurites per cell (n = 3). *p < 0.001, as compared with control group; #p < 0.001, as compared with OGD group; ##p < 0.001, as compared with OGD group; **p < 0.001, as compared with OGD + IL-10 group; by one way analysis of variance (ANOVA) followed by Student-Newman-Keuls multiple comparisons test, F = 305.781, p < 0.0001. For each group and experiment, 20 neurons from 8 ~ 10 randomly selected fields were observed to assess the average length of the longest neurites and average number of primary neurites. Data are presented as mean ± SEM of three independent experiments (n = 3). Scale bar: 20 μm.
