Figure 7
The effect of IL-10 on neurite outgrowth mediated by Netrin-1 after OGD injury.
(A) qPCR analysis of Netrin-1 (n = 3). Control group was set as calibrator sample representing the 1× expression. *p < 0.01, as compared with control group; #p < 0.05, as compared with OGD group; ##p < 0.01, as compared with OGD group; **p < 0.01, as compared with OGD + IL-10 group; by one way analysis of variance (ANOVA) followed by Student-Newman-Keuls multiple comparisons test, F = 27.274, p < 0.0001. (B) The representative image of western blot analysis for Netrin-1. (C) Western blot analysis of Netrin-1 (n = 3). *p < 0.001, as compared with control group; #p < 0.01, as compared with OGD group; ##p < 0.05, as compared with OGD group; **p < 0.05, as compared with OGD + IL-10 group; by one way analysis of variance (ANOVA) followed by Student-Newman-Keuls multiple comparisons test, F = 28.529, p < 0.0001. (D) qPCR analysis of the knockdown efficiency of Netrin-1 (n = 3). Control group was set as calibrator sample representing the 1 × expression. **p < 0.001, as compared with OGD + Scrambled shRNA group; ##p < 0.001, as compared with OGD + Scrambled shRNA + IL-10 group; by one way analysis of variance (ANOVA) followed by Student-Newman-Keuls multiple comparisons test, F = 109.194, p < 0.0001. (E) The representative image of western blot analysis of the knockdown efficiency of Netrin-1. (F) Western blot analysis of the knockdown efficiency of Netrin-1 (n = 5). **p < 0.001, as compared with OGD + Scrambled shRNA group; ##p < 0.001, as compared with OGD + Scrambled shRNA + IL-10 group; by one way analysis of variance (ANOVA) followed by Student-Newman-Keuls multiple comparisons test, F = 173.098, p < 0.0001. (G) Detection of neurites after Netrin-1 knockdown. (a) Control group; (b) OGD group; (c) OGD + scrambled shRNA group; (d) OGD + Netrin-1 shRNA group; (e) OGD + Netrin-1 shRNA + IL-10 group; (f) OGD + scrambled shRNA + IL-10 group; (g) Statistical analysis of the longest neurite length after Netrin-1 knockdown (n = 3). **p < 0.001, as compared with OGD + Scrambled shRNA group; ##p < 0.001, as compared with OGD + Scrambled shRNA + IL-10 group; by one way analysis of variance (ANOVA) followed by Student-Newman-Keuls multiple comparisons test, F = 217.416, p < 0.0001. (h) Statistical analysis of average number of primary neurites after Netrin-1 knockdown (n = 3). **p < 0.001, as compared with OGD + Scrambled shRNA group; ##p < 0.001, as compared with OGD + Scrambled shRNA + IL-10 group; by one way analysis of variance (ANOVA) followed by Student-Newman-Keuls multiple comparisons test, F = 130.618, p < 0.0001. Scale bar: 20 μm. Data are presented as mean ± SEM.
