Figure 8
Synapse formation promoted by IL-10 via JAK1/STAT3 pathway.
(A) qPCR analysis of synaptophysin (n = 3). Control group was set as calibrator sample representing the 1 × expression. *p < 0.001, as compared with control group; #p < 0.05, as compared with OGD group; ##p < 0.001, as compared with OGD group; **p < 0.001, as compared with OGD + IL-10 group; by one way analysis of variance (ANOVA) followed by Student-Newman-Keuls multiple comparisons test, F = 76.701, p < 0.0001. (B) The representative image of western blot analysis for synaptophysin expression. (C) Western blot analysis of synaptophysin (n = 3). *p < 0.01, as compared with control group; #p < 0.001, as compared with OGD group; ##p < 0.01, as compared with OGD group; **p < 0.01, as compared with OGD + IL-10 group; by one way analysis of variance (ANOVA) followed by Student-Newman-Keuls multiple comparisons test, F = 57.656, p < 0.0001. (D) Statistical analysis of the density of dendritic spine (n = 3). *p < 0.001, as compared with control group; #p < 0.001, as compared with OGD group; ##p < 0.001, as compared with OGD group; **p < 0.001, as compared with OGD + IL-10 group; by one way analysis of variance (ANOVA) followed by Student-Newman-Keuls multiple comparisons test, F = 468.104, p < 0.0001. (E) The representative image of excitatory synapses and inhibitory synapses for each group. (F) Statistical analysis of the density of excitatory synapses (n = 3). *p < 0.001, as compared with control group; #p < 0.001, as compared with OGD group; ##p < 0.001, as compared with OGD group; **p < 0.001, as compared with OGD + IL-10 group; by one way analysis of variance (ANOVA) followed by Student-Newman-Keuls multiple comparisons test, F = 227.982, p < 0.0001. (G) Statistical analysis of the density of inhibitory synapses (n = 3). *p < 0.001, as compared with control group; #p < 0.001, as compared with OGD group; ##p < 0.001, as compared with OGD group; **p < 0.001, as compared with OGD + IL-10 group; by one way analysis of variance (ANOVA) followed by Student-Newman-Keuls multiple comparisons test, F = 144.251, p < 0.0001. For each group and experiment, the density of dendritic spines, excitatory synapses and inhibitory synapses were measured from 20 dendrites of eight to ten neurons. Data are presented as mean ± SEM of three independent experiments (n = 3). Scale bar: 10 μm.
