Figure 3
The conversion ratio of L-tert-leucine synthesis reaction catalyzed by the circuits.
(a) 34L-34F (○, e.e value was shown as ▼), mixing B0034 + FDH with B0034 + LeuDH (●, e.e value was shown as ▲); (b) 32L-34F (○, e.e value was shown as ▼), mixing B0034 + FDH with B0032 + LeuDH (◆, e.e value was shown as ▲); (c) 30L-34F (□, e.e value was shown as ▼), mixing B0034 + FDH with B0030 + LeuDH (■, e.e value was shown as ▲); (b) The highest conversion ratio of L-tert-leucine synthesis reaction was catalyzed separately by the 34L-34F, 32L-34F and 30L-34F. Reaction conditions: TMA (100 mM), ammonium formate (200 mM), NADH (0.01 g/L) and cell-free extracts of E. coli cells (4 g/L wet cells) in NH3OH –NH4Cl buffer (1 M, pH 8.0), 37 °C, 220 rpm. All experiments were performed in triplicate and repeated three times. Error bars represent the standard deviation.
