Figure 1
From: Hypoxia induced the differentiation of Tbx18-positive epicardial cells to CoSMCs
Culture of Tbx18-positive epicardial cells from E11.5 Tbx18:Cre/R26REYFP/LacZ tracing hearts.
(A) Direct YFP expression analysis in cryosections of E11.5 Tbx18:Cre/R26REYFP tracing hearts showed that YFP fluorescence was specifically expressed in the epicardium. (B) The β-gal staining of E11.5 hearts from Tbx18:Cre/R26RLacz embryos showed that the epicardium was β-gal-positive. (C) Representative image of Tbx18-positive epicardial cells. (D) YFP expression of Tbx18-positive epicardial cells isolated from Tbx18:Cre/R26REYFP tracing hearts. (E) β-gal-positive staining of cells isolated from Tbx18:Cre/R26RLacZ tracing hearts. (F) Immunostaining for Tbx18 and DAPI nuclear staining of the cultured epicardial cells. (G) qRT-PCR analyses of Tbx18 vs. cTnt (cardiomyocyte-specific marker) expression in Tbx18-positive epicardial cells and cardiomyocytes (p < 0.001). The data are presented as the means ± SD of three experiments, Student’s t test. Scale bars in (A–C) are 100 μm and scale bars in (D–F) are 50 μm.
