Figure 5
From: Hypoxia induced the differentiation of Tbx18-positive epicardial cells to CoSMCs
Expression of major EMT regulators in Tbx18-derived coronary vessels and Tbx18-positive epicardium.
(A) Immunofluorescence for Snail and Slug in Tbx18-positive epicardial-derived CoSMCs of Tbx18:Cre/R26REYFP hearts at E16.5. YFP fluorescence is shown in the Tbx18-positive epicardial-derived cells of Tbx18:Cre/R26REYFP mice. Panels in the lower right corner of (A) represent higher magnification views of the boxed areas in (A) Arrow indicates the merge of Snail with YFP. Arrowhead indicates that Slug did not merge with YFP. Scale bar is 50 μm. (B,C) Immunofluorescence analyses of the Tbx18-lineage reporter YFP and Snail/Slug in E14.5 Tbx18:Cre/R26REYFP embryos after 24 h of maternal hypoxia or normoxia. Arrows and arrowheads indicate the merge of Snail with YFP in the Tbx18-positive epicardium. Scale bar is 50 μm.
