Figure 6
From: Hypoxia induced the differentiation of Tbx18-positive epicardial cells to CoSMCs
Changes in the migration and detachment of the epicardium after hypoxia intervention at E14.5.
(A,B) For the migration analysis, β-gal staining was performed in whole hearts of E14.5 Tbx18:Cre/R26RLacz mice exposed to normoxia (A) or 24 h of maternal hypoxia (B). (C–E) HE stained sections of hearts exposed to normoxia, 3 h of hypoxia and 24 h of hypoxia. Panels in (A’–E’) represent higher magnification views of the boxed areas in (A–E). Arrows in D’ indicate the detached epicardium. Scale bars in (A–E) are 500 μm. Scale bars in A’ and B’ are 50 μm and scale bars in (C’–E’) are 100 μm.
