Figure 4
From: Galangin Induces Autophagy via Deacetylation of LC3 by SIRT1 in HepG2 Cells
Deacetylation of endogenous LC3 by SIRT1 was essential for galangin-induced autophagy.
(A) The binding of endogenous SIRT1 to endogenous LC3 and endogenous LC3 acetylation were detected using co-immunoprecipitation assays and western blot analysis in HepG2 cells treated with DMSO or 130 μM galangin for 18 hours. (B) Endogenous LC3 acetylation was detected by co-immunoprecipitation assays and western blot analysis after HepG2 cells were treated with DMSO or 130 μM galangin in SIRT1-KD, Vector and uninfected cells (Blank).
