Figure 5
TLR4 is involved in RABV-inducted viperin regulation.
(A) Altered TLR4 expression on membranes during RABV infection. RAW264.7 cells were mock-infected, UV-inactivated purified RABV or infected with rRC-HL at an MOI of 0.1 at 37 °C for 24 h and cells were then analysed by flow cytometry. Data from triplicate experiments were then analysed using Guava soft 2.6 software. (B) RAW264.7 cells were infected with rRC-HL at an MOI of 0.1 and were then treated with 1.0 μM or 5.0 μM of TLR4-specific inhibitor TAK-242. The cell lysates were subjected to Western blotting analysis to detect viperin, RABV N protein and β-actin protein expression. (C) The viperin protein/actin ratios in Figure 5D were measured using Li-Cor Odyssey 3.0 analytical software version 29. (D) The RAW264.7 cells were incubated with UV-inactivated purified RABV rRC-HL virion and were then treated with 5.0 μM of TLR4-specific inhibitor TAK-242. The cell lysates were subjected to Western blot analysis to detect viperin, RABV N protein and β-actin protein expression. (E) The viperin/actin ratios in Fig. 5D were measured using Li-Cor Odyssey 3.0 analytical software version 29.
