Figure 6
Identification of cellular factors in the TLR4 signal transduction pathway involved in RABV-inducted viperin regulation.
(A) Detection of cellular factors in the TLR4 signal transduction pathway involved in RABV-induced viperin regulation. RAW264.7 cells were infected with rRC-HL at an MOI of 0.1 and were then used to prepare lysates. These lysates were subjected to Western blotting analysis to detect MyD88, IRF3, IFN-β, viperin, NF-κB (p65), RABV N and β-actin protein expression. (B) Based on (A), the supernatants were used to assess IFN-α/β contents using ELISA kits. (C) The RAW264.7 cells were treated with 2,000 pg/mL IFNβ for 24 h and the cell lysates were then subjected to Western blotting analysis to detect viperin protein expression.
