Table 1 Diet-based results of WCR dsRNA screening.

From: Discovery of midgut genes for the RNA interference control of corn rootworm

dsRNA Name

Length (bp)

Relative to orf

Primary scores

7d LC50, ng μl−1

7d IC50, ng μl−1

Start

End

dvssj1 FIS

1156

−27

1131

2.8

n/a

n/a

dvssj1 frag1

210

−25

185

2.9

0.041

0.013

dvssj1 frag2

145

−6

139

3.0

0.097

0.013

dvssj1 frag5

502

−25

477

2.0

0.082

0.022

dvssj2 FIS

573

2934

3506

3.0

1.699

0.272

dvssj2 frag1

225

3301

3526

2.6

0.286

0.135

dvssj2 frag7

162

16

177

2.4

0.089

0.054

  1. Primary scores were the average of eight observations in cDNA-based first-round IVT screening (FIS) or subsequent fragment screening. LC50 and IC50 values in ng μl−1 during a 7-day assay. Target sequences are indicated relative to the first letter of the start codon ( A TG) of the open-reading frame (orf).
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