Figure 3 | Scientific Reports

Figure 3

From: Warfarin and coumarin-like Murraya paniculata extract down-regulate EpCAM-mediated cell adhesion: individual components versus mixture for studying botanical metastatic chemopreventives

Figure 3

Low cytotoxicity of five components and their effects on adhesion molecule EpCAM of colon cancer cell line HCT116.

(a) MTT assay showed low cytotoxicity of the five components (Z1~Z5), their mixture (CM) and warfarin against HCT116 when their concentrations reached 500 μg/mL and beyond. (b) quantitative flow cytometric analysis revealed concentration-dependent effects of Z1~Z5, CM and warfarin (<100 μg/mL) on expression of EpCAM on HCT116; the results demonstrated that warfarin, CM, Z3, Z4 and Z5 down-regulated EpCAM expression, while Z1 and Z2 did oppositely. (c) flow cytometric scanning of effects of warfarin, CM and Z1-Z5 on EpCAM expression using the isotype as the control. Bars represent the mean ± SEM (n = 3). *P < 0.05 and **P < 0.01, compared with the control.

Back to article page