Figure 5

The change of oocytes meiosis and relative gene expressions in untreated, Cy3-siRNA and Mfn2-siRNA groups.
(A) Representative images of spindle and chromosome alignment immunostained with anti-α-tubulin monoclone antibody (green) and Hochest 33258 (blue) by using confocal microscope in the three groups (Arrows, abnormal chromosome alignment and spindle with few microtubules); (B) The relative expression levels of meiosis-related genes DAZL and SCP3 in the three groups. *P < 0.05 vs. Untreated and Cy3-siRNA groups.