Figure 6
Dis-assembly studies of Sup35-NM fibrils.
(A) Electrophoregram for FP and AG fibrils under SDS treatment. Lane 1: directly loaded FP fibril; lane 2: FP fibril under SDS; lane 3: FP fibril under SDS-boiling; lane 4: directly loaded AG fibril; lane 5: AG fibril under SDS; lane 6: AG fibril under SDS-boiling; lane M: molecular weight protein marker comprising 116 kDa, 66.2 kDa, 45 kDa, 35 kDa, 25 kDa, and 18.4 kDa. (B) Electrophoregram for ST fibril (2-day) under SDS treatment. Lane 1: directly loaded ST fibril; lanes 2–9: ST-fibril under SDS-boiling (0.55%, 1.1%, 1.65%, 2.2%, 3.3%, 4.4%, 5.5%, 6.6%); lane 10: ST-fibril under SDS (6.6%). (C) Percentage of ATmer in 1-month (Fig. 6A, lane 6) and 2-month (Figure S6, lane 4) AG fibrils. Student’s t-test: **P < 0.01 (mean ± s.d.). (D) Percentage of ATmer in 2-day ST fibril after being stored at 4 °C for different number of days (0 day: Fig. 6B, lanes 7, 8, 9 or Figure S7A, lane 4; 2 days: Figure S7B, lane 2; 5 days: Figure S7C, lane 3). Student’s t-test: **P < 0.01 (mean ± s.d.). (E) SEC curves for FP fibril (blue), AG fibril (red) and Sup35-NM monomer (green) under SDS-boiling treatment. (F) Electrophoregram for AG fibril under SDS treatment. 41 min species (Figure S8, red): directly loaded (lane 1) and under SDS-boiling (lane 2); 47 min species (Fig. 6E, red): directly loaded (lane 3) and under SDS-boiling (lane 4); 66 min species (Fig. 6E, red): directly loaded (lane 5) and under SDS-boiling (lane 6).
