Figure 1 | Scientific Reports

Figure 1

From: Peptides interfering with protein-protein interactions in the ethylene signaling pathway delay tomato fruit ripening

Figure 1

Interaction studies of tomato ETR1 and EIN2 via MST.

Determination of Kd value of LeEIN2-LeETR1 complex formation based on MST-data is illustrated. Normalized fluorescence as MST signal is plotted against increasing LeEIN2462–1316 (●) concentrations as described. Binding curve was calculated by a model assuming one binding site per binding partner and resulted in a Kd = 619 nM +/− 176 nM. As negative control thermally and chemically denaturated LeEIN2462–1316 (○) was used. The MST signal indicates no interaction with the purified LeETR1 receptor. All data represent the mean +/− standard deviation.

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